phospho-C-REL (Ser503) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12918R-FITC-100uL |
| Product Name | phospho-C-REL (Ser503) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human C-REL around the phosphorylation site of Ser503 |
| Modification Site | Ser503 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5966 |
| Swiss Prot | Q04864 |
| Subcellular location | Nucleus |
| Synonyms | c-Rel phospho S503; c-Rel phospho Ser503; p-c-Rel phospho S503; p-c-Rel S503; Avian reticuloendotheliosis; C REL; C Rel protein; c Rel proto oncogene protein; Oncogene REL; Oncogene REL avian reticuloendotheliosis; REL; v rel avian reticuloendotheliosis viral oncogene homolog; v rel reticuloendotheliosis viral oncogene homolog. |
| Background | The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM, May 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-C-REL (Ser503) Polyclonal Antibody, FITC Conjugated (bs-12918R-FITC) validated for, and at what dilution?
bs-12918R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-C-REL (Ser503) Polyclonal Antibody, FITC Conjugated (bs-12918R-FITC) react with?
Tested reactivity: Human.
How should bs-12918R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-C-REL (Ser503) Polyclonal Antibody, FITC Conjugated (bs-12918R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human C-REL around the phosphorylation site of Ser503
What are the excitation and emission wavelengths of the FITC conjugate (bs-12918R-FITC)?
FITC on bs-12918R-FITC: excitation / emission 494nm/518nm.