phospho-CK18 (Ser52) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12921R-BF750-100uL |
| Product Name | phospho-CK18 (Ser52) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse CK18 around the phosphorylation site of Ser52 [SR(p-S)VW] |
| Modification Site | Ser52 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 16668 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Keratin, type I cytoskeletal 18; Cytokeratin endo B; Keratin D; Cytokeratin-18; CK-18; Keratin-18; K18; Krt18; Kerd; Krt1-18 |
| Background | When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CK18 (Ser52) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12921R-BF750) validated for, and at what dilution?
bs-12921R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CK18 (Ser52) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12921R-BF750) react with?
Tested reactivity: Mouse, Rat.
How should bs-12921R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CK18 (Ser52) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12921R-BF750)?
KLH conjugated synthetic phosphopeptide derived from mouse CK18 around the phosphorylation site of Ser52 [SR(p-S)VW]
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-12921R-BF750)?
AbBy Fluor-750 on bs-12921R-BF750: excitation / emission 749nm/775nm.