CYP26A1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12928R-BF405 |
| Product Name | CYP26A1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP26A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1592 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CP26; CP26A_HUMAN; CYP26; CYP26A 1; cyp26a1; Cytochrome P450 26A1; Cytochrome P450 family 26 subfamily A polypeptide 1; Cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, family 26, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIA, polypeptide 1; Cytochrome P450RAI; hP450RAI; OTTHUMP00000020102; OTTHUMP00000020103; P450; P450 retinoic acid inactivating 1; P450RAI; P450RAI1; Retinoic acid 4 hydroxylase; Retinoic acid 4-hydroxylase; Retinoic acid metabolizing cytochrome; Retinoic acid-metabolizing cytochrome. |
| Background | The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP26A1 is a major retinoic acid catabolic enzyme. CYP26A1 plays an important role in protecting tailbud tissues from inappropriate exposure to retinoic acid. CYP26A1 transcription is epigenetically regulated by nuclear retinoic acid receptor 2. Mutations in the gene encoding for CYP26A1 are associated with caudal agenesis and spina bifida, imperforate anus, agenesis of the caudal portions of the digestive and urogenital tracts, and malformed lumbosacral skeletal elements. CYP26A1 is upregulated in adenomatous polyposis coli mouse adenomas, human FAP adenomas, human sporadic colon carcinomas, and in the intestine of adenomatous polyposis coli (apcmcr) mutant zebrafish embryos. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYP26A1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-12928R-BF405) validated for, and at what dilution?
bs-12928R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP26A1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-12928R-BF405) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-12928R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP26A1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-12928R-BF405)?
KLH conjugated synthetic peptide derived from human CYP26A1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-12928R-BF405)?
AbBy Fluor-405 on bs-12928R-BF405: excitation / emission 401nm/421nm.