CTNS Polyclonal Antibody

$Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human SW480 cell lysates; Lane 9: Human 293T cell lysates probed with CTNS Polyclonal Antibody, Unconjugated (bs-12932R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Muscle tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human SW480 cell lysates; Lane 9: Human 293T cell lysates probed with CTNS Polyclonal Antibody, Unconjugated (bs-12932R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Rabbit

Overview
Catalog #	bs-12932R
Product Name	CTNS Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CTNS/Cystinosin
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1497
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CTNS LSB; Cystinosin; cystinosis, nephropathic; PQLC4; CTNS_HUMAN.
Background	Cystinosis is an autosomal recessive disorder resulting from defective lysosomal transport of cystine and present at birth as a failure to thrive, rickets and proximal renal tubular acidosis. The human CTNS gene on chromosome 17p13 encodes the protein Cystinosin, and mutations in CTNS are responsible for nephropathic cystinosis. The CTNS promoter contains an Sp1 binding element. Cystinosin is an integral membrane protein containing 7 transmembrane domains that functions as a H+-driven transporter responsible for cystine export from lysosomes. In humans, Cystinosin is expressed abundantly in pancreas, kidney (mature and fetal), and skeletal muscle. The mouse homolog to CTNS encodes a protein which is expressed in all tissues except skeletal muscle. In the cell, Cystinosin co-localizes with LAMP-2 to lysosomes. A C-terminal GYDQL sorting motif within Cystinosin is critical for lysosomal localization.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200