CSPS/MPST Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12945R-BF647 |
| Product Name | CSPS/MPST Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CSPS |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 101060714 |
| Subcellular location | Cytoplasm |
| Synonyms | Aryl sulfotransferase 1A3/1A4; Aryl sulfotransferase; Catecholamine Sulfating Phenol Sulfotransferase; Catecholamine-sulfating phenol sulfotransferase; Dopamine specic sulfotransferase; FLJ54864; HAST; HAST3; M PST; M-PST; Monoamine preferring sulfotransferase; Monoamine sulfating phenosulfotransferase; monoamine-preferring; Monoamine-sulfating phenol sulfotransferase; MPST; Phenol sulfotransferase 1A5; Placental estrogen sulfotransferase; ST1A3/ST1A4; ST1A3_HUMAN; ST1A5; STM; Sulfokinase; Sulfotransferase 1A3/1A4; Sulfotransferase; Sulfotransferase family cytosolic 1A phenol preferring member 3; Sulfotransferase family cytosolic 1A phenol preferring member 4; Sulfotransferase monoamine preferring; SULT1A 3; SULT1A4; Thermolabile monoamine M form phenol sulfotransferase; Thermolabile phenol sulfotransferase; TL PST; TL-PST; TLPST. |
| Background | Cysteine string proteins (CSPs) are synaptic vesicle-associated, secretory vesicle proteins that are involved in Ca2+-regulated exocytosis of synaptic vesicles and modulation of presynaptic transmembrane calcium fluxes in neuroendocrine and endocrine cell types. CSP contains a J-domain that binds HSP 70/HSC 70 chaperone ATPases and a membrane-targeting, palmitoylated cysteine-rich string region. CSPs may act as molecular chaperones in synapses, and mediate conformational folding of components of the vesicular exocytotic machinery. CSP is involved in the fine tuning of neurotransmission through its interaction with receptor-coupled trimeric GTP binding proteins (G proteins) and N-type Ca2+ channels. Two variants of CSP have been described: CSP1; and the 31 amino acid, C-terminally truncated isoform, CSP2. Subcellular fractionation of insulinoma cells shows CSP1 in granular fractions, while the membrane and cytosol fractions contain predominantly CSP2. The fractions also contain additional proteins, presumably CSP dimers. Furthermore, in various mammalian cell lines (including rat brain) CSP1 expression predominates CSP2 expression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CSPS/MPST Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12945R-BF647) validated for, and at what dilution?
bs-12945R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CSPS/MPST Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12945R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Rabbit.
How should bs-12945R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CSPS/MPST Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12945R-BF647)?
KLH conjugated synthetic peptide derived from human CSPS
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12945R-BF647)?
AbBy Fluor-647 on bs-12945R-BF647: excitation / emission 650nm/665nm.