CRTAC1 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12948R-BF555 |
| Product Name | CRTAC1 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRTAC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55118 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | 68 kDa chondrocyte expressed protein; 68 kDa chondrocyte-expressed protein; Acidic secreted protein in cartilage; ASPIC; ASPIC1; Cartilage acidic protein 1; Cartilage acidic protein 1 precursor; CEP 68; CEP-68; CEP68; CRAC1_HUMAN; CRTAC1; Protein CRTAC1-B; W307. |
| Background | CRTAC1 is a 661 amino acid secreted protein. CRTAC1 is O-glycosylated and contains one EGF-like domain and four FG-GAP repeats. Up-regulated in mesenchymal stem cells undergoing chondrogenic differentiation and also by growth factor BMP-4, CRTAC1 is found in the interterritorial matrix of articular deep zone cartilage. CRTAC1 is expressed as three isoforms produced by alternative splicing events. All isoforms are expressed in bone, cartilage and lung, while isoforms 1 and 2 are expressed in the brain. The gene that encodes CRTAC1 maps to human chromosome 10, which contains over 800 genes and 135 million nucleotides, making up nearly 4.5% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
Frequently asked questions
Which applications is CRTAC1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12948R-BF555) validated for, and at what dilution?
bs-12948R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CRTAC1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12948R-BF555) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep.
How should bs-12948R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CRTAC1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-12948R-BF555)?
KLH conjugated synthetic peptide derived from human CRTAC1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-12948R-BF555)?
AbBy Fluor-555 on bs-12948R-BF555: excitation / emission 553nm/568nm.