phospho-Acetyl Coenzyme A Carboxylase beta (Ser220) Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12952R-Biotin-100uL |
| Product Name | phospho-Acetyl Coenzyme A Carboxylase beta (Ser220) Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Acetyl Coenzyme A Carboxylase beta around the phosphorylation site of Ser220 |
| Modification Site | Ser220 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Swiss Prot | O00763 |
| Subcellular location | Cell membrane |
| Synonyms | Acetyl Coenzyme A Carboxylase beta phospho S220; Acetyl Coenzyme A Carboxylase beta phospho Ser2210 p-Acetyl Coenzyme A Carboxylase beta phospho S220; ACACB_HUMAN |
| Background | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-Acetyl Coenzyme A Carboxylase beta (Ser220) Polyclonal Antibody, Biotin Conjugated (bs-12952R-Biotin) validated for, and at what dilution?
bs-12952R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12952R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Acetyl Coenzyme A Carboxylase beta (Ser220) Polyclonal Antibody, Biotin Conjugated (bs-12952R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human Acetyl Coenzyme A Carboxylase beta around the phosphorylation site of Ser220