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Defensin beta 2 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Rat
Overview
Catalog # bs-1296R-PerCP-100uL
Product Name Defensin beta 2 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from Rat Beta-defensin 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 641631
Subcellular location Secreted
Synonyms Beta-defensin 2; Defb2; Beta-defensin 4
Background Beta-defensin 2 (BD-2) also known as skin-antimicrobial peptide 1 (SAP1) is a peptide that is encoded by the DEFB4 (defensin, beta 4) gene. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. Beta-defensin 2 is an antibiotic peptide which is locally regulated by inflammation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Defensin beta 2 Polyclonal Antibody, PerCP Conjugated (bs-1296R-PerCP) validated for, and at what dilution?

bs-1296R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Defensin beta 2 Polyclonal Antibody, PerCP Conjugated (bs-1296R-PerCP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-1296R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Defensin beta 2 Polyclonal Antibody, PerCP Conjugated (bs-1296R-PerCP)?

KLH conjugated synthetic peptide derived from Rat Beta-defensin 2

What are the excitation and emission wavelengths of the PerCP conjugate (bs-1296R-PerCP)?

PerCP on bs-1296R-PerCP: excitation / emission 482nm/677nm.