CD28 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Lane 1: Thymus lysates probed with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.