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    CD28 Polyclonal Antibody

    Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated(bs-1297R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
    Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
    Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
    Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
    Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
    Description:
    Applications:
    • FCM
    • IHC-P
    • IF(IHC-P)
    This product has been cited in the published journals, click to see.
    Overview
    Catalog # bs-1297R
    Product Name CD28 Polyclonal Antibody
    Applications FCM, IHC-P, IF(IHC-P)
    Reactivity Human, Mouse, Rat
    Specifications
    Conjugation Unconjugated
    Host Rabbit
    Source KLH conjugated synthetic peptide derived from mouse CD28
    Immunogen Range 20-45/218
    Clonality Polyclonal
    Isotype IgG
    Concentration 1ug/ul
    Purification Purified by Protein A.
    Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
    Target
    Gene ID 12487
    Swiss Prot P31041
    Subcellular location Extracellular, Cell membrane
    Synonyms T-cell-specific surface glycoprotein CD28; CD28
    Background Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
    Applications
    Application Dilution
    FCM 1:20-100
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200