phospho-DAP Kinase 2 (Ser318) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12980R-BF350-100uL |
| Product Name | phospho-DAP Kinase 2 (Ser318) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human DAP Kinase 2 around the phosphorylation site of Ser318 |
| Modification Site | Ser318 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23604 |
| Subcellular location | Cytoplasm |
| Synonyms | DAP Kinase 2 phospho S318; p-DAP Kinase 2 phospho S318; Death associated protein kinase 2; DAK 2; DAK2; DAP kinase related protein 1; DAPK 2; DAPK2; Death associated protein kinase 2; DRP 1; DRP1; DRP-1; MGC119312; DAP kinase 2; DAP kinase related protein 1; DAP kinase related protein 1 beta isoform; DAP-kinase-related protein 1; DAPK 2; DAPK2; DAPK2_HUMAN; Death associated protein kinase 2; Death-associated protein kinase 2; DRP 1; DRP-1; DRP1; MGC119312. |
| Background | Protein kinase termed (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-DAP Kinase 2 (Ser318) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12980R-BF350) validated for, and at what dilution?
bs-12980R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12980R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DAP Kinase 2 (Ser318) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12980R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human DAP Kinase 2 around the phosphorylation site of Ser318
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-12980R-BF350)?
AbBy Fluor-350 on bs-12980R-BF350: excitation / emission 346nm/442nm.