Help

DAPL1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Horse
Overview
Catalog # bs-12981R-BF350-100uL
Product Name DAPL1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Horse
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DAPL1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 92196
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms dapL1; DAPL1_HUMAN; death associated protein-like 1; Death-associated protein-like 1; Early epithelial dferentiation-associated protein; EEDA; RP23-151F21.4.
Background In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved cytoplasmic region called the death domain?. DAPL1 (Death-associated protein-like 1), also known as EEDA (Early epithelial differentiation-associated protein), is a 107 amino acid protein that is expressed in hair follicles and is thought to function in a similar manner to DAP-1, possibly participating in the early stages of epithelial differentiation and/or apoptosis.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DAPL1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12981R-BF350) validated for, and at what dilution?

bs-12981R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DAPL1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12981R-BF350) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Horse.

How should bs-12981R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DAPL1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-12981R-BF350)?

KLH conjugated synthetic peptide derived from human DAPL1

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-12981R-BF350)?

AbBy Fluor-350 on bs-12981R-BF350: excitation / emission 346nm/442nm.