phospho-delta 1 Catenin (Tyr228) Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12992R-PerCP-100uL |
| Product Name | phospho-delta 1 Catenin (Tyr228) Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human delta 1 Catenin around the phosphorylation site of Tyr228 |
| Modification Site | Tyr228 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1500 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | delta 1 Catenin phospho Y228; p-delta 1 Catenin phospho Y228; Cadherin associated Src substrate; Cadherin-associated Src substrate; CAS; Catenin cadherin associated protein delta 1; Catenin delta 1; Catenin delta-1; CTND1_HUMAN; CTNND 1; CTNND; CTNND1; KIAA0384; p120; P120 CAS; p120 catenin; P120 CTN; p120Cas; p120Ctn; P120CAS; P120CTN. |
| Background | Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-delta 1 Catenin (Tyr228) Polyclonal Antibody, PerCP Conjugated (bs-12992R-PerCP) validated for, and at what dilution?
bs-12992R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-delta 1 Catenin (Tyr228) Polyclonal Antibody, PerCP Conjugated (bs-12992R-PerCP) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-12992R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-delta 1 Catenin (Tyr228) Polyclonal Antibody, PerCP Conjugated (bs-12992R-PerCP)?
KLH conjugated synthetic phosphopeptide derived from human delta 1 Catenin around the phosphorylation site of Tyr228
What are the excitation and emission wavelengths of the PerCP conjugate (bs-12992R-PerCP)?
PerCP on bs-12992R-PerCP: excitation / emission 482nm/677nm.