Dermcidin Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12996R-RBITC-100uL |
| Product Name | Dermcidin Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Dermcidin/DCD |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 117159 |
| Subcellular location | Secreted |
| Synonyms | AIDD; DCD 1; dcd; DCD-1; DCD_HUMAN; DSEP; HCAP; P; Preproteolysin. |
| Background | Antimicrobial peptides participate in the innate response, which may provide a barrier for protection against infection. The Dermcidin gene encodes an antimicrobial peptide DCD-1, which is constitutively expressed in sweat glands, secreted into the sweat, and transported to the epidermal surface. DCD-1 displays antimicrobial activity in response to a variety of pathogenic microorganisms. Overexpression of Dermcidin in breast cancers promotes cell growth and survival, and is coupled with a focal copy number gain of its locus on human chromosome 12q13.2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Dermcidin Polyclonal Antibody, RBITC Conjugated (bs-12996R-RBITC) validated for, and at what dilution?
bs-12996R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12996R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Dermcidin Polyclonal Antibody, RBITC Conjugated (bs-12996R-RBITC)?
KLH conjugated synthetic peptide derived from human Dermcidin/DCD
What are the excitation and emission wavelengths of the RBITC conjugate (bs-12996R-RBITC)?
RBITC on bs-12996R-RBITC: excitation / emission 540nm/625nm.