Parvalbumin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

$Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at room temperature for 30 minutes; Antibody incubation with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with Parvalbumin Polyclonal Antibody, Unconjugated (bs-1299R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-1299r
Product Name	Parvalbumin Polyclonal Antibody
Applications	WB, ELISA, IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Parvalbumin Intermedial
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5816
Swiss Prot	P20472
Subcellular location	Nucleus, Extracellular matrix
Synonyms	D22S749; MGC116759; Parvalbumin alpha; PRVA_HUMAN; PVALB.
Background	The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Belongs to the parvalbumin family. & Contains 2 EF-hand domains. This protein binds two calcium ions.
Application Dilution
WB
ELISA	1:500-1000
IF(ICC)	1:50-200