POLK Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13019R-APC-Cy7-100uL |
| Product Name | POLK Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNA Polymerase Kappa/POLK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51426 |
| Subcellular location | Nucleus |
| Synonyms | DNA polymerase kappa; DINB protein; Short=DINP; POLK_HUMAN. |
| Background | DNA polymerase lambda (pol Kappa), also designated DNA polymerase Kappa or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol Kappa is a member of the DNA polymerase type-X family. Pol Kappa extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol Kappa may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol Kappa and thus influences the ability of pol Kappa to synthesize DNA. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is POLK Polyclonal Antibody, APC-Cy7 Conjugated (bs-13019R-APC-Cy7) validated for, and at what dilution?
bs-13019R-APC-Cy7 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does POLK Polyclonal Antibody, APC-Cy7 Conjugated (bs-13019R-APC-Cy7) react with?
Tested reactivity: Human.
How should bs-13019R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise POLK Polyclonal Antibody, APC-Cy7 Conjugated (bs-13019R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human DNA Polymerase Kappa/POLK
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-13019R-APC-Cy7)?
APC-Cy7 on bs-13019R-APC-Cy7: excitation / emission 650nm/780nm.