DNA Polymerase lambda Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13020R-PerCP-100uL |
| Product Name | DNA Polymerase lambda Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNA Polymerase lambda |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27343 |
| Subcellular location | Nucleus |
| Synonyms | BETA N; BETAN; DNA directed DNA polymerase lambda; DNA polymerase beta 2; DNA polymerase beta-2; DNA polymerase kappa; DNA polymerase kappa DNA polymerase beta N; DNA polymerase lambda; DNA polymerase lamda2; DPOLL_HUMAN; EC 2.7.7.7,EC 4.2.99.; FLJ46002; OTTHUMP00000020321; OTTHUMP00000020323; OTTHUMP00000059179; Pol beta2; POL KAPPA; Pol Lambda; POLKAPPA; POLL; Polymerase DNA directed lambda. |
| Background | DNA polymerase lambda (pol lambda), also designated DNA polymerase lambda or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol lambda is a member of the DNA polymerase type-X family. Pol lambda extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ?may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol lambda and thus influences the ability of pol ?to synthesize DNA. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DNA Polymerase lambda Antibody, PerCP Conjugated (bs-13020R-PerCP) validated for, and at what dilution?
bs-13020R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNA Polymerase lambda Antibody, PerCP Conjugated (bs-13020R-PerCP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Chicken, Rabbit.
How should bs-13020R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNA Polymerase lambda Antibody, PerCP Conjugated (bs-13020R-PerCP)?
KLH conjugated synthetic peptide derived from human DNA Polymerase lambda
What are the excitation and emission wavelengths of the PerCP conjugate (bs-13020R-PerCP)?
PerCP on bs-13020R-PerCP: excitation / emission 482nm/677nm.