DNA polymerase mu Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13021R-BF647-100uL |
| Product Name | DNA polymerase mu Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNA polymerase mu |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27434 |
| Subcellular location | Nucleus |
| Synonyms | DNA Polymerase Mu; DNA-directed DNA polymerase mu; DNA-directed DNA/RNA polymerase mu; DPOLM_HUMAN; FLJ35482; Pol iota; Pol Mu; Polm; POLM protein; Polymerase DNA directed mu; Polymerase DNA directed mu; Tdt N; Tdt-N; TdtN; Terminal transferase. |
| Background | DNA polymerase mu shares a number of characteristics with DNA polymerase Beta as well as with terminal deoxynucleotideyltransferase. Pol mu purportedly plays a role in microhomology mediated joining and the repair of double-stranded breaks. However, unlike other DNA polymerases, which show substrate specificity for deoxynucleotides, DNA Pol mu incorporates both deoxynucleotides and ribonucleotides in a template- directed manner. This unusual capability implies a novel role for this polymerase in DNA repair. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DNA polymerase mu Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-13021R-BF647) validated for, and at what dilution?
bs-13021R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNA polymerase mu Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-13021R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Horse.
How should bs-13021R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNA polymerase mu Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-13021R-BF647)?
KLH conjugated synthetic peptide derived from human DNA polymerase mu
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-13021R-BF647)?
AbBy Fluor-647 on bs-13021R-BF647: excitation / emission 650nm/665nm.