DNAJC9 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13024R-BF750-100uL |
| Product Name | DNAJC9 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNAJC9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23234 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane, Extracellular matrix |
| Synonyms | DnaJ homolog subfamily C member 9; DnaJ Hsp40 homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9_HUMAN; HDJC9; J DOMAIN OF DNAJ LIKE PROTEIN 1; JDD1; SB73. |
| Background | The DnaJ family comprises a group of chaperone proteins that contain a J domain and have diverse cellular localization and functions. DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis and are also important mediators of proteolysis and protein degradation. DnaJC9 (DnaJ (Hsp40) homolog, subfamily C, member 9), also designated HDJC9, JDD1 or DnaJ protein SB73, is a 260 amino acid protein found at moderate levels in most tissues with highest expression in the germinal zone of the central nervous system, testis, ovary, renal cortex and fetal liver. A member of the DnaJ family, DnaJC9 contains one N-terminal J domain but lacks the typical G/F and zinc finger regions that are typical of DnaJ family members. DnaJC9 localiizes to nuclei under normal conditions but may be transported to cytoplasm and plasma membrane when exposed to heat shock. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DNAJC9 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13024R-BF750) validated for, and at what dilution?
bs-13024R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNAJC9 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13024R-BF750) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Rabbit.
How should bs-13024R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNAJC9 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-13024R-BF750)?
KLH conjugated synthetic peptide derived from human DNAJC9
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-13024R-BF750)?
AbBy Fluor-750 on bs-13024R-BF750: excitation / emission 749nm/775nm.