DTYMK Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13034R-Cy5-100uL |
| Product Name | DTYMK Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DTYMK/CDC8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1841 |
| Subcellular location | Cytoplasm |
| Synonyms | CDC8; Deoxythymidylate kinase thymidylate kinase; deoxythymidylate kinase; dTMP kinase; Dtymk; FLJ44192; KTHY_HUMAN; MGC198617; OTTHUMP00000164604; OTTHUMP00000200145; OTTHUMP00000200146; PP3731; Thymidylate kinase; TMPK; TYMK. |
| Background | DTYMK is a 212 amino acid protein that belongs to the thymidylate kinase family and is involved in pyrimidine metabolism. Specifically, DTYMK catalyzes the ATP-dependent conversion of dTMP (deoxythymidine monophosphate) to dTDP (deoxythymidine diphosphate), which then functions as one of the four nucleotides in DNA. Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth. DTYMK expression levels peak during the S phase (synthesis phase) of the cell cycle, further supporting the role of DTYMK in DNA synthesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DTYMK Polyclonal Antibody, Cy5 Conjugated (bs-13034R-Cy5) validated for, and at what dilution?
bs-13034R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13034R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DTYMK Polyclonal Antibody, Cy5 Conjugated (bs-13034R-Cy5)?
KLH conjugated synthetic peptide derived from human DTYMK/CDC8
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13034R-Cy5)?
Cy5 on bs-13034R-Cy5: excitation / emission 625,650nm/670nm.