SFRP1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1303R-Cy5.5-100uL |
| Product Name | SFRP1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SFRP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6422 |
| Swiss Prot | Q8N474 |
| Subcellular location | Secreted |
| Synonyms | FRP; FRP1; FrzA; FRP-1; SARP2; Secreted frizzled-related protein 1; sFRP-1; Secreted apoptosis-related protein 2; SARP-2; SFRP1 |
| Background | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SFRP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1303R-CY5-5) validated for, and at what dilution?
BS-1303R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SFRP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1303R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-1303R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SFRP1 Polyclonal Antibody, Cy5.5 Conjugated (BS-1303R-CY5-5)?
KLH conjugated synthetic peptide derived from human SFRP1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-1303R-CY5-5)?
Cy5.5 on BS-1303R-CY5-5: excitation / emission 675nm/694nm.