EBP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13047R-BF680-100uL |
| Product Name | EBP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EBP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5036 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | AA672939; Cell cycle protein p38 2G4 homolog; Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1; ErbB3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; hG4 1; hG4-1; IRES-specic cellular trans-acting factor 45 kDa; MGC81621; MGC94070; Mpp1; p38 2G4; PA2G4; PA2G4_HUMAN; Plfap; proliferation associated 2G4; proliferation associated 2G4, 38-KD; proliferation-associated 2G4, 38kDa; proliferation-associated 2G4, a; proliferation-associated protein 1; proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732. |
| Background | EBP1 is a member of the peptidase M24C family and functions as an RNA-binding protein involved in cellular proliferation and differentiation processes. It is expressed in a variety of cell lines, including a wide range of tumor cell lines, and localizes to the cytoplasm. Upon treatment with Neuregulin-1 (heregulin), EBP1 translocates to the nucleus. EBP1 is a component of pre-ribosomal ribonucleoprotein complexes, participating in ribosome assembly and regulating the later steps of rRNA processing. In addition, EBP1 interacts with ErbB-3 and may function as a modulator of the ErbB-3-mediated signal transduction pathway by regulating the effects of Neuregulin-1 (heregulin). EBP1 also associates with histone deacetylases (HDACs), functioning as a transcriptional co-repressor of cell cycle regulatory genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EBP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13047R-BF680) validated for, and at what dilution?
bs-13047R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EBP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13047R-BF680) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Chicken, Rabbit, Zebrafish.
How should bs-13047R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EBP1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13047R-BF680)?
KLH conjugated synthetic peptide derived from human EBP1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-13047R-BF680)?
AbBy Fluor-680 on bs-13047R-BF680: excitation / emission 679nm/702nm.