ECH1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13048R-BF350-100uL |
| Product Name | ECH1 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ECH1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1891 |
| Subcellular location | Cytoplasm |
| Synonyms | 4-dienoyl-CoA isomerase; 5-Delta2; Delta3 5 Delta2 4 dienoyl CoA isomerase; Delta3 5 Delta2 4 dienoyl CoA isomerase mitochondrial; Delta3; Delta3 5 delta2 4 dienoyl CoA isomerase; Dienoyl CoA isomerase; ECH 1; Ech1; ECH1_HUMAN; Enoyl Coenzyme A hydratase 1; Enoyl Coenzyme A hydratase 1 peroxisomal; HPXEL; mitochondrial; MS1034; Peroxisomal enoyl CoA hydratase 1; Peroxisomal enoyl coenzyme A hydratase like protein. |
| Background | ECH1 is a 328 amino acid protein that localizes to both the mitochondrion and the peroxisome and belongs to the hydratase/isomerase superfamily. Existing as a homohexamer, ECH1 is involved in the fatty acid-beta oxidation pathway, specifically functioning to catalyze the isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. The gene encoding ECH1 maps to human chromosome 19, which is the genetic home for a number of immunoglobulin superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcRs). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ECH1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13048R-BF350) validated for, and at what dilution?
bs-13048R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ECH1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13048R-BF350) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-13048R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ECH1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-13048R-BF350)?
KLH conjugated synthetic peptide derived from human ECH1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-13048R-BF350)?
AbBy Fluor-350 on bs-13048R-BF350: excitation / emission 346nm/442nm.