EED Polyclonal Antibody, Cy3 Conjugated

Applications

WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Xenopus laevis
Xenopus tropicalis
Medaka fish

Predicted Reactivity

Human
Mouse
Rat
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Zebrafish

Overview
Catalog #	bs-13053R-Cy3
Product Name	EED Polyclonal Antibody, Cy3 Conjugated
Applications	WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Xenopus laevis, Xenopus tropicalis, Medaka fish
Predicted Reactivity	Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation	Cy3
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EED
Immunogen Range	51-150/441
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	8726
Subcellular location	Nucleus
Synonyms	eed; EED protein; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1.
Background	The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5% identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins.
Application Dilution
WB	1:300-5000
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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