eEF1A2 Polyclonal Antibody

$Mouse heart and brain lysates probed with Rabbit Anti-eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.$

Mouse heart and brain lysates probed with Rabbit Anti-eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$25 ug total protein per lane of various lysates (see on figure) probed with eEF1A2 polyclonal antibody, unconjugated (bs-13054R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with eEF1A2 polyclonal antibody, unconjugated (bs-13054R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Zebrafish

Overview
Catalog #	bs-13054R
Product Name	eEF1A2 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity	The antibody may recognize both eEF1A1 and eEF1A2 due to high sequence similarity
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human eEF1A2
Immunogen Range	1-100/463
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1915, 1917
Subcellular location	Nucleus
Synonyms	eEF1A1: Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7 eEF1A2: Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1
Background	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200