eEF1A2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13054R-Cy5.5-100uL |
| Product Name | eEF1A2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | The antibody may recognize both eEF1A1 and eEF1A2 due to high sequence similarity |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Zebrafish |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eEF1A2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1915, 1917 |
| Subcellular location | Nucleus |
| Synonyms | eEF1A1: Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7 eEF1A2: Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1 |
| Background | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eEF1A2 Polyclonal Antibody, Cy5.5 Conjugated (BS-13054R-CY5-5) validated for, and at what dilution?
BS-13054R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eEF1A2 Polyclonal Antibody, Cy5.5 Conjugated (BS-13054R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Zebrafish.
How should BS-13054R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eEF1A2 Polyclonal Antibody, Cy5.5 Conjugated (BS-13054R-CY5-5)?
KLH conjugated synthetic peptide derived from human eEF1A2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-13054R-CY5-5)?
Cy5.5 on BS-13054R-CY5-5: excitation / emission 675nm/694nm.