EEF1D Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13055R-100uL |
| Product Name | EEF1D Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EEF1D |
| Immunogen Range | 1-100/281 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1936 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; Eukaryotic translation elongation factor 1 delta; Guanine nucleotide exchange protein. |
| Background | This gene encodes a subunit of the elongation factor-1complex, which is responsible for the enzymatic delivery ofaminoacyl tRNAs to the ribosome. This subunit, delta, functions asguanine nucleotide exchange factor. It is reported that followingHIV-1 infection, this subunit interacts with HIV-1 Tat. Thisinteraction results in repression of translation of host cellproteins and enhanced translation of viral proteins. Severalalternatively spliced transcript variants encoding multipleisoforms have been found for this gene. Related pseudogenes havebeen defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided byRefSeq, Aug 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EEF1D Polyclonal Antibody (bs-13055R) validated for, and at what dilution?
bs-13055R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EEF1D Polyclonal Antibody (bs-13055R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-13055R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EEF1D Polyclonal Antibody (bs-13055R)?
KLH conjugated synthetic peptide derived from human EEF1D Immunogen range: 1-100/281.