EEF1D Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13055R-BF594-100uL |
| Product Name | EEF1D Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EEF1D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1936 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; Eukaryotic translation elongation factor 1 delta; Guanine nucleotide exchange protein. |
| Background | This gene encodes a subunit of the elongation factor-1complex, which is responsible for the enzymatic delivery ofaminoacyl tRNAs to the ribosome. This subunit, delta, functions asguanine nucleotide exchange factor. It is reported that followingHIV-1 infection, this subunit interacts with HIV-1 Tat. Thisinteraction results in repression of translation of host cellproteins and enhanced translation of viral proteins. Severalalternatively spliced transcript variants encoding multipleisoforms have been found for this gene. Related pseudogenes havebeen defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided byRefSeq, Aug 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EEF1D Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13055R-BF594) validated for, and at what dilution?
bs-13055R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EEF1D Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13055R-BF594) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-13055R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EEF1D Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13055R-BF594)?
KLH conjugated synthetic peptide derived from human EEF1D
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-13055R-BF594)?
AbBy Fluor-594 on bs-13055R-BF594: excitation / emission 590nm/617nm.