Phospho-EEF2 (Thr56 + Thr58) Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13057R-APC-Cy5.5 |
| Product Name | Phospho-EEF2 (Thr56 + Thr58) Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human EEF2 around the phosphorylation site of Thr56+Thr58 |
| Modification Site | Thr56 + Thr58 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1938 |
| Subcellular location | Cytoplasm |
| Synonyms | EEF2 phospho T56 + T58; p-EEF2 phospho T56 + T58; EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase. |
| Background | EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-EEF2 (Thr56 + Thr58) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-13057R-APC-CY5-5) validated for, and at what dilution?
BS-13057R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-EEF2 (Thr56 + Thr58) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-13057R-APC-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should BS-13057R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-EEF2 (Thr56 + Thr58) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-13057R-APC-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human EEF2 around the phosphorylation site of Thr56+Thr58
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-13057R-APC-CY5-5)?
APC-Cy5.5 on BS-13057R-APC-CY5-5: excitation / emission 650nm/694nm.