Phospho-eNOS (Ser632) Polyclonal Antibody, PerCP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13074R-PerCP-100uL |
| Product Name | Phospho-eNOS (Ser632) Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser633 |
| Modification Site | Ser633 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4846 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | eNOS phospho S633; p-eNOS phospho S633; eNOS 633; cNOS; eNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
| Background | eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-eNOS (Ser632) Polyclonal Antibody, PerCP Conjugated (bs-13074R-PerCP) validated for, and at what dilution?
bs-13074R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13074R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-eNOS (Ser632) Polyclonal Antibody, PerCP Conjugated (bs-13074R-PerCP)?
KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser633
What are the excitation and emission wavelengths of the PerCP conjugate (bs-13074R-PerCP)?
PerCP on bs-13074R-PerCP: excitation / emission 482nm/677nm.