ENT1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13077R-BF680 |
| Product Name | ENT1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Horse, Orangutan |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ENT1/SLC29A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2030 |
| Subcellular location | Cell membrane |
| Synonyms | Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside NBMPR-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; SLC29A1; solute carrier family 29 nucleoside transporters, member 1; Solute carrier family 29 member 1. |
| Background | Equilibrative nucleoside transporters (ENTs) regulate many physiological processes and are widely distributed in mammals, plants, yeasts, insects, nematodes and protozoans. They enable facilitated diffusion of hydrophilic nucleosides, such as adenosine and nucleoside analogs, across cell membranes. ENTs are required for uptake of antiviral and anticancer nucleoside drugs and influence a variety of physiological processes, such as neurotransmission and platelet aggregation, by regulating the amount of adenoside available to cell surface receptors. Equilibrative nucleoside transporter 1 (ENT1), also designated solute carrier family 29 (nucleoside transporters), member 1, belongs to the SLC29A transporter family and is a mammalian ENT isoform. ENT1, along with ENT3, mediates the majority of influx and efflux of nucleosides across the plasma membrane. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ENT1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13077R-BF680) validated for, and at what dilution?
bs-13077R-BF680 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ENT1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13077R-BF680) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Horse, Orangutan.
How should bs-13077R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ENT1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-13077R-BF680)?
KLH conjugated synthetic peptide derived from human ENT1/SLC29A1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-13077R-BF680)?
AbBy Fluor-680 on bs-13077R-BF680: excitation / emission 679nm/702nm.