Phospho-HER2 (Tyr1221) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13090R-APC |
| Product Name | Phospho-HER2 (Tyr1221) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Dog |
| Predicted Reactivity | Mouse, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HER2 around the phosphorylation site of Tyr1221 |
| Modification Site | Tyr1221 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2064 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ErbB 2 phospho Y1221; p-ErbB 2 phospho Y1221; HER2Tyr1221; ErbB2; CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; CD340 antigen; Cerb B2/neu protein; Erb B2; erbb2; HER 2; HER 2/neu; HER2; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; Neuroblastoma/glioblastoma derived oncogene homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1; ERBB2_HUMAN. |
| Background | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-HER2 (Tyr1221) Polyclonal Antibody, APC Conjugated (bs-13090R-APC) validated for, and at what dilution?
bs-13090R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-HER2 (Tyr1221) Polyclonal Antibody, APC Conjugated (bs-13090R-APC) react with?
Tested reactivity: Human, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig, Horse, Rabbit, GuineaPig.
How should bs-13090R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HER2 (Tyr1221) Polyclonal Antibody, APC Conjugated (bs-13090R-APC)?
KLH conjugated synthetic phosphopeptide derived from human HER2 around the phosphorylation site of Tyr1221
What are the excitation and emission wavelengths of the APC conjugate (bs-13090R-APC)?
APC on bs-13090R-APC: excitation / emission 650nm/660nm.