ERCC8 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-ERCC8\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-ERCC8\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Mouse testis lysates probed with Rabbit Anti-ERCC*\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.$

Mouse testis lysates probed with Rabbit Anti-ERCC*\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Lane 1: Liver lysates probed with ERCC8\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Liver lysates probed with ERCC8\/CSA Polyclonal Antibody, Unconjugated (bs-13095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Horse

Overview
Catalog #	bs-13095R
Product Name	ERCC8 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ERCC8/CSA
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1161
Swiss Prot	Q13216
Subcellular location	Nucleus
Synonyms	CSA; CKN1; UVSS2; DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA; ERCC8
Background	Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200