Epsin 1/EPN1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13101r-bf405 |
| Product Name | Epsin 1/EPN1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Epsin 1/EPN1 |
| Immunogen Range | 130-175/576 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29924 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | EH domain binding mitotic phosphoprotein; EPN 1; EPN-1; EPS 15 interacting protein 1; EPS-15-interacting protein 1; Epsin 1; EPN1_HUMAN. |
| Background | Epsin 1 is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the ?subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both Epsin and Eps15, like other cytosolic components of the synaptic vesicle endo-cytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an Epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Epsin 1/EPN1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13101R-BF405) validated for, and at what dilution?
bs-13101R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Epsin 1/EPN1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13101R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-13101R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Epsin 1/EPN1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-13101R-BF405)?
KLH conjugated synthetic peptide derived from human Epsin 1/EPN1 Immunogen range: 130-175/576.
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-13101R-BF405)?
AbBy Fluor-405 on bs-13101R-BF405: excitation / emission 401nm/421nm.