ESRP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13108R-BF594-100uL |
| Product Name | ESRP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ESRP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80004 |
| Subcellular location | Nucleus |
| Synonyms | Epithelial splicing regulatory protein 2; ESRP 2; ESRP2; ESRP2_HUMAN; FLJ21918; FLJ22248; RBM35B; RNA binding mot protein 35A; RNA binding mot protein 35B; RNA binding protein 35B; RNA-binding mot protein 35B; RNA-binding protein 35B. |
| Background | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ESRP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13108R-BF594) validated for, and at what dilution?
bs-13108R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ESRP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13108R-BF594) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow.
How should bs-13108R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ESRP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-13108R-BF594)?
KLH conjugated synthetic peptide derived from human ESRP2
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-13108R-BF594)?
AbBy Fluor-594 on bs-13108R-BF594: excitation / emission 590nm/617nm.