phospho-ETS1 (Thr38) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13113R-Cy3-100uL |
| Product Name | phospho-ETS1 (Thr38) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ETS1 around the phosphorylation site of Thr38 |
| Modification Site | Thr38 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2113 |
| Subcellular location | Nucleus |
| Synonyms | ETS1 phospho T38; ETS1 phospho Thr38; p-ETS1 phospho T38; Avian erythroblastosis virus E26 v ets oncogene homolog 1; C ets 1 protein; ETS 1; Ets protein; ETS1; ETS-1; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; p54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1. |
| Background | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-ETS1 (Thr38) Polyclonal Antibody, Cy3 Conjugated (bs-13113R-Cy3) validated for, and at what dilution?
bs-13113R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ETS1 (Thr38) Polyclonal Antibody, Cy3 Conjugated (bs-13113R-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bs-13113R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ETS1 (Thr38) Polyclonal Antibody, Cy3 Conjugated (bs-13113R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human ETS1 around the phosphorylation site of Thr38
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13113R-Cy3)?
Cy3 on bs-13113R-Cy3: excitation / emission 512,550nm/570,615nm.