LYVE-1 Polyclonal Antibody

$Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LYVE-1 Polyclonal Antibody, Unconjugated (bs-1311R) at 1:600 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bs-1311r-TR
Product Name	LYVE-1 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse LYVE-1 terminus
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10894
Swiss Prot	Q8BHC0
Subcellular location	Cytoplasm, Cell membrane, Extracellular matrix
Synonyms	LYVE1; lymphalic vessel endotheilial hyaluronan receptor 1; CRSBP 1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; HAR; hyaluronic acid receptor; Cell surface retention sequence-binding protein 1; CRSBP-1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1_MOUSE; Lymphatic endothelium specific hyaluronan receptor; LYVE 1; LYVE-1; XLKD1.
Application Dilution
IHC-P	=1:200-500
IHC-F	IHC-F=1:200-500
IF	IF=1:200-500