EXOSC10/PMSCL2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human skin labeled with Anti-EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n$

Formalin-fixed and paraffin embedded human skin labeled with Anti-EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n

$Formalin-fixed and paraffin embedded mouse lymph node labeled with Anti-EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n$

Formalin-fixed and paraffin embedded mouse lymph node labeled with Anti-EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n

$U937 cell lysates probed with EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

U937 cell lysates probed with EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EXOSC10\/PMSCL2 Polyclonal Antibody, Unconjugated (bs-13120R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-13120R
Product Name	EXOSC10/PMSCL2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EXOSC10/PMSCL2
Immunogen Range	41-140/885
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5394
Subcellular location	Cytoplasm, Nucleus
Synonyms	Autoantigen PM/Scl 2; Exosc10; Exosome component 10; EXOSX_HUMAN; P100 polymyositis scleroderma overlap syndrome associated autoantigen; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; p2; p3; p4; PM Scl; PM/Scl 100; PM/Scl-100; PMSCL; PMSCL2; Polymyositis/scleroderma autoantigen 100 kDa; Polymyositis/scleroderma autoantigen 2 100 kDa; Polymyositis/scleroderma autoantigen 2; RRP6; Rrp6p.
Background	The exosome is a multi-subunit complex composed of several highly conserved proteins, some of which are 3? to 5? exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3? region. EXOSC10, also known as PMSCL, PMSCL2, p2, p3, p4, RRP6, Rrp6p, PM-Scl, or PM/Scl-100, is an 885 amino acid protein that contains one HRDC domain and one 3?-5? enonuclease domain. Localized to both the cytoplasm and the nucleus, EXOSC10 is part of the post-splicing exosome complex and is involved in mRNA surveillance, mRNA nuclear export and nonsense-mediated decay of mRNAs containing premature stop codons. against EXOSC10 have been found in patients with scleroderma and/or polymyositis (chronic diseases of the skin and muscle, respectively), suggesting that EXOSC10 may be involved in the pathogenesis of these diseases. Two isoforms of EXOSC10 exist due to alternative splicing events.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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