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Factor D Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-13130R-PerCP-100uL
Product Name Factor D Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Factor D/Adipsin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1675
Swiss Prot P00746
Subcellular location Secreted
Synonyms DF; ADN; PFD; ADIPSIN; Complement factor D; C3 convertase activator; Properdin factor D; CFD
Background Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Factor D Polyclonal Antibody, PerCP Conjugated (bs-13130R-PerCP) validated for, and at what dilution?

bs-13130R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Factor D Polyclonal Antibody, PerCP Conjugated (bs-13130R-PerCP) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-13130R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Factor D Polyclonal Antibody, PerCP Conjugated (bs-13130R-PerCP)?

KLH conjugated synthetic peptide derived from human Factor D/Adipsin

What are the excitation and emission wavelengths of the PerCP conjugate (bs-13130R-PerCP)?

PerCP on bs-13130R-PerCP: excitation / emission 482nm/677nm.