FAN1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-13137R-Cy7 |
| Product Name | FAN1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAN1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22909 |
| Swiss Prot | Q9Y2M0 |
| Subcellular location | Nucleus |
| Synonyms | KMIN; hFAN1; MTMR15; KIAA1018; Fanconi-associated nuclease 1; FANCD2/FANCI-associated nuclease 1; Myotubularin-related protein 15; FAN1 |
| Background | Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is FAN1 Polyclonal Antibody, Cy7 Conjugated (bs-13137R-Cy7) validated for, and at what dilution?
bs-13137R-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAN1 Polyclonal Antibody, Cy7 Conjugated (bs-13137R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-13137R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAN1 Polyclonal Antibody, Cy7 Conjugated (bs-13137R-Cy7)?
KLH conjugated synthetic peptide derived from human FAN1
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-13137R-Cy7)?
Cy7 on bs-13137R-Cy7: excitation / emission 743nm/767nm.