FAU Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13148R-HRP-100uL |
| Product Name | FAU Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAU |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2197 |
| Swiss Prot | P62861 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 40S ribosomal protein S30; asr1; FAU; FAU encoded ubiquitin like protein; FAU1; FBR MuSV associated ubiquitously expressed; Finkel Biskis Reilly murine sarcoma virus FBR MuSV ubiquitously expressed fox derived; Finkel Biskis Reilly murine sarcoma virus FBR MuSV ubiquitously expressed; Finkel Biskis Reilly murine sarcoma virus ubiquitously expressed; FLJ22986; Fub1; Fubi; MNSFbeta; Monoclonal nonspecic suppressor factor beta; Ribosomal protein S30; RPS30; S30; UBIM_HUMAN; Ubiquitin like protein fubi and ribosomal protein S30; Ubiquitin like protein fubi; Ubiquitin like S30 fusion protein; Ubiquitin-like protein FUBI. |
| Background | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. A wide range of enzymes facilitate in the proteolytic Ub pathway, including monoclonal nonspecific suppressor factor-beta (MNSF-beta), a subunit of MNSF, which is a lymphokine product of a murine T cell hybrid-oma that restricts the production of LPS-induced immunoglobulin secreting cells in an antigen-nonspecific manner. MNSF-beta is a ubiquitin-like fusion protein consisting of the ribosomal protein S30 and a protein that shares 36% sequence identity with ubiquitin. This ubiquitin-like segment (Ubi-L) can be cleaved from MNSF-beta in the cytosol. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is FAU Polyclonal Antibody, HRP Conjugated (bs-13148R-HRP) validated for, and at what dilution?
bs-13148R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13148R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAU Polyclonal Antibody, HRP Conjugated (bs-13148R-HRP)?
KLH conjugated synthetic peptide derived from human FAU