FBXO45 Polyclonal Antibody

Mouse intestine lysates probed with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin-embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin-embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin-embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.

$Lane 1: Human A431 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human Jurkat cell lysates probed with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Human A431 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human Jurkat cell lysates probed with FBXO45 Polyclonal Antibody, Unconjugated (bs-13150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-13150r-TR
Product Name	FBXO45 Polyclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FBXO45
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	200933
Swiss Prot	P0C2W1
Subcellular location	Cell membrane
Synonyms	F box protein 45; F-box only protein 45; F-box/SPRY domain-containing protein 1; FBSP1_HUMAN; FBX45; fbxo45; hFbxo45.
Application Dilution
WB	=1:500-2000