Fibulin 2 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13160R-PE-Cy5 |
| Product Name | Fibulin 2 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Fibulin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2199 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Fibulin-2; Fbln2; FBLN2_HUMAN; FIBL-2; Fibulin-2; OTTHUMP00000216670; OTTHUMP00000216671; OTTHUMP00000216672. |
| Background | Fibulin-1 and Fibulin-2 associate with Fibronectin and other extracellular matrix proteins. In bone marrow, Fibulin-1 and Fibulin-2 bind to Fibronectin in the adherent layer. Fibulin-1 expression is stimulated by estrogen in ovarian cancer cell lines and has been suggested as both an agent of metastasis in ovarian cancer cells and as an indicator for predicting cancer risk or aggressiveness in ovarian carcinomas. The mobility of cancer cells may be inhibited with increasing exposure to Fibulin-1. Fibulin-2 binds to the lectin domains of extracellular matrix proteins aggrecan, versican and brevican. Fibulin-2 is abundantly expressed in heart, placenta and ovarian tissue, where it localizes to basement membranes and connective tissue compartments. In mice, differential Fibulin-2 gene expression correlates with the early phase of diabetic kidneys and glomerulosclerosis. The gene encoding human Fibulin-2 maps to chromosome 3p25.1. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Fibulin 2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-13160R-PE-Cy5) validated for, and at what dilution?
bs-13160R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13160R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fibulin 2 Polyclonal Antibody, PE-Cy5 Conjugated (bs-13160R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Fibulin 2
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-13160R-PE-Cy5)?
PE-Cy5 on bs-13160R-PE-Cy5: excitation / emission 488nm/670nm.