Fibulin 7 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13161R-Cy5.5-100uL |
| Product Name | Fibulin 7 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Pig, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Fibulin 7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 129804 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | FBLN7; FBLN7_HUMAN; FIBL-7; Fibl7; Fibulin-7; TM14. |
| Background | Fibulin-7 is a 439 amino acid extracellular matrix protein that belongs to the Fibulin family. Containing two EGF-like domains and one sushi (CCP/SCR) domain, Fibulin-7 exists as four alternatively spliced isoforms. Fibulin-7 is considered an adhesion protein that interacts with extracellular matrix molecules in developing teeth, and may be involved in differentiation and maintenance of odontoblasts as well as in dentin formation. Fibulin-7 is post-translationally glycosylated with N-linked oligosaccharides and interacts with heparin, fibronectin, fibulin-1 and DSP (dentin sialophosphoprotein). Fibulin-7 is encoded by a gene located on human chromosome 2, which consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Fibulin 7 Polyclonal Antibody, Cy5.5 Conjugated (BS-13161R-CY5-5) validated for, and at what dilution?
BS-13161R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-13161R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fibulin 7 Polyclonal Antibody, Cy5.5 Conjugated (BS-13161R-CY5-5)?
KLH conjugated synthetic peptide derived from human Fibulin 7
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-13161R-CY5-5)?
Cy5.5 on BS-13161R-CY5-5: excitation / emission 675nm/694nm.