FbxL4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13166R-Cy5.5-100uL |
| Product Name | FbxL4 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Sheep, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FbxL4/FBL4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26235 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | F box and leucine rich repeat protein 4; F box protein FBL4/FBL5; F box/LRR repeat protein 4; F-box and leucine-rich repeat protein 4; F-box protein FBL 4; F-box protein FBL4/FBL5; F-box/LRR-repeat protein 4; FBL 4; FBL 5; FBL4; FBL5; Fbxl4; FBXL4_HUMAN. |
| Background | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 9 tandem leucine-rich repeats. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FbxL4 Polyclonal Antibody, Cy5.5 Conjugated (BS-13166R-CY5-5) validated for, and at what dilution?
BS-13166R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FbxL4 Polyclonal Antibody, Cy5.5 Conjugated (BS-13166R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Sheep, Chicken.
How should BS-13166R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FbxL4 Polyclonal Antibody, Cy5.5 Conjugated (BS-13166R-CY5-5)?
KLH conjugated synthetic peptide derived from human FbxL4/FBL4
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-13166R-CY5-5)?
Cy5.5 on BS-13166R-CY5-5: excitation / emission 675nm/694nm.