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PDGFBB Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1316R-FITC-100uL
Product Name PDGFBB Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PDGF-B
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5155
Subcellular location Secreted
Synonyms PDGF BB; PDGF-BB; platelet derived growth factor-BB; Becaplermin; C sis; FLJ12858; Oncogene SIS; PDG B chain; PDGB; PDGF 2; PDGF B; PDGF B chain; PDGF2; PDGFB; PDGFB/COL1A1 fusion gene; Platelet derived growth factor 2; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide; Simian sarcoma viral v sis oncogene homolog; SIS; SSV; V sis platelet derived growth factor beta polypeptide.
Background Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PDGFBB Polyclonal Antibody, FITC Conjugated (bs-1316R-FITC) validated for, and at what dilution?

bs-1316R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PDGFBB Polyclonal Antibody, FITC Conjugated (bs-1316R-FITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1316R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PDGFBB Polyclonal Antibody, FITC Conjugated (bs-1316R-FITC)?

KLH conjugated synthetic peptide derived from human PDGF-B

What are the excitation and emission wavelengths of the FITC conjugate (bs-1316R-FITC)?

FITC on bs-1316R-FITC: excitation / emission 494nm/518nm.

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