phospho-FGFR3 (Tyr724) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13170R-Cy5 |
| Product Name | phospho-FGFR3 (Tyr724) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FGFR3 around the phosphorylation site of Tyr724 |
| Modification Site | Tyr724 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2261 |
| Swiss Prot | P22607 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | FGFR3 phospho Y724; FGFR3 phospho Tyr724; p-FGFR3 phospho Y724; ACH; CD 333; CD333; CD333 antigen; CEK 2; CEK2; FGFR 3; Fibroblast growth factor receptor 3 achondroplasia thanatophoric dwarfism; Fibroblast growth factor receptor 3; Heparin binding growth factor receptor; HSFGFR3EX; Hydroxyaryl protein kinase; JTK 4; JTK4; MFR 3; SAM 3; Tyrosine kinase JTK 4; Tyrosine kinase JTK4; Z FGFR 3. |
| Background | The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is phospho-FGFR3 (Tyr724) Polyclonal Antibody, Cy5 Conjugated (bs-13170R-Cy5) validated for, and at what dilution?
bs-13170R-Cy5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-FGFR3 (Tyr724) Polyclonal Antibody, Cy5 Conjugated (bs-13170R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Rabbit.
How should bs-13170R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-FGFR3 (Tyr724) Polyclonal Antibody, Cy5 Conjugated (bs-13170R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human FGFR3 around the phosphorylation site of Tyr724
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-13170R-Cy5)?
Cy5 on bs-13170R-Cy5: excitation / emission 625,650nm/670nm.