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FMO5 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
Overview
Catalog # bs-13187R-Cy3-100uL
Product Name FMO5 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FMO5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2330
Swiss Prot P49326
Subcellular location Cytoplasm, Cell membrane
Synonyms Dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; Hepatic flavin-containing monooxygenase 5; FMO 5; FMO5
Background In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FMO5 Polyclonal Antibody, Cy3 Conjugated (bs-13187R-Cy3) validated for, and at what dilution?

bs-13187R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FMO5 Polyclonal Antibody, Cy3 Conjugated (bs-13187R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.

How should bs-13187R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FMO5 Polyclonal Antibody, Cy3 Conjugated (bs-13187R-Cy3)?

KLH conjugated synthetic peptide derived from human FMO5

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13187R-Cy3)?

Cy3 on bs-13187R-Cy3: excitation / emission 512,550nm/570,615nm.