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IL-5 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti- IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti- IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1318R
Product Name IL-5 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse IL-5
Immunogen Range 56-132/132
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition Store at -20°C for 12 months..
Target
Gene ID 16191
Swiss Prot P04401
Subcellular location Secreted
Synonyms Il-5; Interleukin-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF; Il5
Background Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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