FOXF2 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13201R-FITC-100uL |
| Product Name | FOXF2 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FOXF2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2295 |
| Subcellular location | Nucleus |
| Synonyms | FKH L6; FKHL 6; FKHL6; Forkhead box F2; Forkhead box protein F2; Forkhead like 6; Forkhead related activator 2; Forkhead related protein FKHL6; Forkhead related transcription factor 2; FOX F2; FOXF 2; FREAC 2; FREAC2; OTTHUMP00000017863; |
| Background | The FOX family of transcription factors share a common DIUA binding domain termed a winged-helix or forkhead domain. Many FOX proteins play important roles in development, metabolism, cancer and aging. Development of the vertebrate gut is controlled by paracrine crosstalk between the endodermal epithelium and the associated splanchnic mesoderm. FOXF2 (forkhead box F2), also known as FKHL or FREAC2, is expressed in lung and placenta, and has been shown to transcriptionally activate several lung-specific genes. FOXF2 interacts with transcription factors TFIIB and TBP, and may be involved in regulating transcription in embryogenesis and pattern formation in multicellular organisms. FOXF2-deficient mice develop cleft palate and an abnormal tongue, which suggests that FOXF2 may be critical for palatogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FOXF2 Polyclonal Antibody, FITC Conjugated (bs-13201R-FITC) validated for, and at what dilution?
bs-13201R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FOXF2 Polyclonal Antibody, FITC Conjugated (bs-13201R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Chicken.
How should bs-13201R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FOXF2 Polyclonal Antibody, FITC Conjugated (bs-13201R-FITC)?
KLH conjugated synthetic peptide derived from human FOXF2
What are the excitation and emission wavelengths of the FITC conjugate (bs-13201R-FITC)?
FITC on bs-13201R-FITC: excitation / emission 494nm/518nm.