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Phospho-FoxO1 (Ser329) Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-13207R-FITC-100uL
Product Name Phospho-FoxO1 (Ser329) Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Ser329
Modification Site Ser329
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2308
Subcellular location Cytoplasm, Nucleus
Synonyms FOXO1A phospho S329; p-FOXO1A S329; FKHRSer319; Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A.
Background This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Phospho-FoxO1 (Ser329) Polyclonal Antibody, FITC Conjugated (bs-13207R-FITC) validated for, and at what dilution?

bs-13207R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-FoxO1 (Ser329) Polyclonal Antibody, FITC Conjugated (bs-13207R-FITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-13207R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-FoxO1 (Ser329) Polyclonal Antibody, FITC Conjugated (bs-13207R-FITC)?

KLH conjugated synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Ser329

What are the excitation and emission wavelengths of the FITC conjugate (bs-13207R-FITC)?

FITC on bs-13207R-FITC: excitation / emission 494nm/518nm.